IODINE MASH & TEST
GUIDE
"You stand at the crossroads of body and fermentability. One drop reveals whether your starch has surrendered to enzymes or is still hiding in the husks."
No Guesswork.
The iodine mash test gives you X-ray vision into your mash. Mash conversion isn’t a guarantee. Temperature drift, pH swings, and enzyme limits can stall breakdown. Unconverted starch steals fermentable sugars, clogs filters, and muddies foam.
This simple chemical reaction rings the alarm, allowing you to tweak rest time or temperature to lock in yield, clarity, and consistent body before you sparge.
The Detection Limit
Iodine slips into the 1.5-nanometer-wide amylose helix, forming a polyiodide complex that absorbs light at 580nm.
Topic 1: Molecular Kinetics
Amylose, Amylopectin, and Iodine Intercalation
The Polymer Challenge
Grain starch is stored in microscopic granules of two polymers: linear amylose and highly branched amylopectin. Beta-amylase cleaves maltose units from chain ends, while Alpha-amylase attacks internal bonds to yield dextrins. Enzyme kinetics follow Michaelis-Menten dynamics, with a Vmax tied directly to temperature and pH stability.
Beta-Amylase Window
Peaks at pH 5.2 - 5.4. Creates fermentable maltose.
Alpha-Amylase Window
Peaks at pH 5.6 - 5.8. Creates body-building dextrins.
Topic 2: Lab Apparatus
Precision Tools for Accurate Readings
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Topic 3: Execution Logic
Step-by-Step Verification Process
The Thermal Sampling
At the end of your rest, draw 5-10 mL of wort. Crucial: Cool the sample below 40°C. Iodine reacts unpredictably at high temperatures, often sublimating or failing to complex with starch, leading to false negatives (amber result despite starch presence).
The Reaction Event
Add exactly 0.05 mL of iodine solution. Observe immediately (within 30 seconds).
Corrective Action
If starch remains, raise mash temp by 2°C or extend the rest by 10 minutes and retest. Repeat until the amber endpoint is reached. Never proceed to boil with a positive starch test unless you desire haze and instability.
Topic 4: Troubleshooting
Solving for Persistent Starch
Crush Mechanics
Persistent blue often means uneven particle size. If the crush is too coarse, endosperm remains trapped behind husks where enzymes cannot reach. Adjust your mill gap by 0.1mm increments to expose more surface area without shredding husks.
Ion Stabilization
Check water hardness. Excess calcium stabilizes alpha-amylase, protecting it from thermal denaturation. If calcium is too low ({'<'}50ppm), enzyme activity wanes rapidly, leading to stalled conversion even at correct temperatures.
Advanced Insight: Beta-Glucanase
Step mashes change granule gelatinization. An early cereal mash at 50-55°C activates beta-glucanase to break cell walls. If you push high-adjunct bills (rice/corn), the iodine test is critical to confirming that barley enzymes can handle the extra load.
VISUALIZE
Precision
IN THE MASH
"Enzyme supplementation can rescue underperforming mashes, but only if you know conversion stalled in the first place. Use the iodine test to validate your mash schedule. Stop guessing, start measuring."